Journal: BMC Veterinary Research

Article Title: Long-term changes of Th17 and regulatory T cells in peripheral blood of dogs with spinal cord injury after intervertebral disc herniation

doi: 10.1186/s12917-023-03647-8

Figure Lengend Snippet: Illustration of a Treg and a Th17 cell analysis using flow cytometry Results of Treg and Th17 cell analysis of a dog after recovery of spinal cord injury using MACSQuant analysis software In the prior performed isotype controls, limits were set at approx. 2% of the cells [ , – ]. This limit was transferred to the test fractions (pink lines) in each case to detect the percentages of FOXP3 (a) or IL17 (b) positive cells, which are located to the right of the pink line Absolute cell numbers were subsequently calculated to make the analyses comparable a) The graphic displays the percentage of FOXP3 positive cells for detection of Treg cells. 9.56% FOXP3 positive cells were identified b) The graphic displays the percentage of IL17 positive cells for detection of Th17. 16.20% IL17 positive cells were identified. The results of a stimulated Th17 cell fraction are shown

Article Snippet: For cell fixation, a flow cytometry fixation buffer (R&D Systems®, Minneapolis, Minnesota, USA) was used.

Techniques: Cell Analysis, Flow Cytometry, Software